Peter M. Haney, MD, PhD
Assistant Professor of Pediatrics/Section of Neonatology
Baylor College of Medicine
phaney@bcm.tmc.edu
Attending Physician
Section of Neonatology
Texas Children's Hospital
B.S. University of Scranton, Scranton, Pennsylvania
Ph.D., Biochemistry, Case Western Reserve University, Cleveland,
Ohio
M.D. Case Western Reserve University School of Medicine, Cleveland,
Ohio
Regulation of Glucose Transport in Lactating
Mammary Gland
The long-term goal of my research is to understand the molecular
cell biology of lactation.
Current work is focused on glucose transport in lactating mammary
gland. Since glucose is the key substrate for the synthesis
of lactose, the major osmotic constituent of human milk, it is the
major determinant of the volume of milk produced. Glucose
also is a key substrate for the synthesis of lipid, the major determinant
of the caloric concentration of human milk. I am studying
the regulation of the amount, activity, and subcellular targeting
of GLUT1, the only glucose transporter isoform identified in mammary
gland, in established and primary mammary epithelial cell lines
as well as in humans and rodents. Efforts are underway to
elucidate the mechanisms of altered glucose transporter targeting,
including Golgi sequestration and polarization of plasma membrane
distribution, that we have observed during lactation and to understand
the impact of GLUT1 retargeting and of altered GLUT1 gene expression
on lactose synthesis and milk production. An artificial rearing
system to study nutrient-gene interactions and possible programming
effects in the regulation of glucose transport during rodent development
.
Representative Publications:
Haney PM, Levy MA, Strube MS, Mueckler M. Insulin-sensitive targeting
of the GLUT4 glucose transporter in L6 myoblasts is conferred by
its COOH-terminal cytoplasmic tail. J Cell Biol 1995; 129:641-658.
Nemeth BA, Tsang SWY, Geske, RS, Haney PM. Golgi targeting of the
GLUT1 glucose transporter in lactating mouse mammary gland. Pediatr
Res 2000; 47:444-450.
Haney, P.M. Targeting of the GLUT1 glucose transporter to Brefeldin
A-sensitive vesicles of CIT3 mouse mammary epithelial cells. Cell
Biology Internat. 2001; 25:277-288.
Abstracts
Riskin, A, Nannegari, VH, Haney, PM. GLUT1 colocalizes with the
proteins of lactose synthetase and with endosomes in human mammary
epithelial cells. Ped Res 2000; 47:296A (SPR poster presentation).
Riskin, A, Nannegari, VH, Haney, PM. An intact endosomal pathway
is required for normal targeting of GFP-GLUT1 glucose transporter
fusion proteins in living mouse mammary epithelial cells. Ped Res
2000; 47:296A (SPR poster symposium presentation).
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