David M. Cohen, Ph.D.
Assistant Professor of Pediatrics
Baylor College of Medicine
dcohen@bcm.tmc.edu
Nutrition, Metabolism and Modeling
I am interested in the regulated coordination of metabolic fluxes
that is fundamental to health and sustained by adequate nutrition.
Study of the quantitative relationships among metabolic flux rates
depends on accurate measurement of those rates, preferably in vivo.
To this end, I have investigated mathematical aspects of modeling
rates of metabolic pathways, subsequent to the administration of
isotopically labeled precursors. The goals of this research
are to improve accuracy as well as to obtain simpler methods for
estimation of metabolic rates. I am currently applying these
formulas in experiments investigating the regulation of metabolic
fluxes associated with energy production in heart cells.
I have a longstanding interest in questions related to the use
of isotopic labeling patterns of metabolic intermediates (i.e.,
positional isotopomers) for the purpose of estimation of rates of
metabolic flux. For this purpose, I developed what I believe
to be a novel approach to modeling and computer simulation of isotopomers.
I am currently pursuing studies in cerebral amino acid metabolism
in which the relationship between positional isotopomers and 13C
nuclear magnetic resonance spectroscopy of tissue extracts will
be exploited.
An important focus of my work is the measurement of cerebral metabolism
in vivo, using nuclear magnetic resonance spectroscopy. Currently,
I am developing new methods for estimation of the rate of cerebral
glucose metabolism, with the goal of improving time resolution.
Additionally, I am interested in neuronal-glial metabolic interactions,
as they may be inferred from measurements in vivo. In the
long term, I hope to learn more about the role of diet in support
of brain metabolism and function.
Representative publications:
Wei, Jingna, Cohen, David M., Quast, Michael J. Effects of 2-Deoxy-D-glucose
on focal cerebral ischemia in hyperglycemic rats. J. Cerebral Blood
Flow Metab. (in press).
Cohen, David M., Wei, Jingna, Smith, E. O’Brian, Gao, Xiaolian,
Quast, Michael J., and Sokoloff, Louis. A Method for Measuring Cerebral
Glucose Metabolism In Vivo by 13C-NMR Spectroscopy. Magnetic Resonance
in Medicine, 48, 1063-1067, 2002.
Goodwin, Gary W., Cohen, David M., and Taegtmeyer, Heinrich. [5-3H]glucose
overestimates glycolytic flux in isolated working rat heart: role
of the pentose phosphate pathway. Am. J. Physiol. Endocrinol. Metab.,
280, E502-508, 2001.
Gotoh, Jun, Kuang, Tang-Yong, Nakao, Yasuaki, Cohen, David M.,
Melzer, Peter, Itoh, Yoshiaki, Pak, Hazel, Pettigrew, Karen D.,
and Sokoloff, Louis. Regional differences in mechanisms of cerebral
circulatory response to neuronal activation. Am. J. Physiol. Heart
Circ. Physiol., 280, H821-H829,2001.
Nakao, Yasuaki, Gotoh, Jun, Kuang, Tang-Yong, Cohen, David M.,
Pettigrew, Karen D., and Sokoloff, Louis. Cerebral blood flow responses
to somatosensory stimulation are unaffected by scopolamine in unanesthetized
rat. J. Pharmacol. Experimental Therapeutics, 290 (2), 929-934,
1999.
Cohen, David M. and Bergman, Richard N. Improved Estimation of
Anaplerosis in Heart Using 13C-NMR. Am. J. Physiol., 273:E1228-E1242,
1997.
Finch, Caleb E. and Cohen, David M. Aging, Metabolism, and Alzheimer
Disease: Review and Hypotheses. Experimental Neurology, 143:82-102,
1997.
|
|
