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Fellows |
 Mike Janeczko, Ph.D.
B.S. Fordham University (Biology),
1997
M.D. University of Medicine and Dentistry
of New Jersey , New Jersey Medical School, 2001
The amino acid glutamate is a component of dietary
protein and also common additive in many food preparations. Our
past studies have shown that glutamate is a major oxidative
fuel for intestinal epithelial cells and is extensively
metabolized by the gut. The aim of my research
project is to further elucidated the capacity of the
gut to metabolize glutamate and the potential effects
of glutamate on other aspects of gastrointestinal physiology.
 John Stephens, M.D.
MD University of South Florida, Tampa,
Florida 1999
BS University of Miami, Coral Gables, Florida 1995
I was originally exposed to research
in my college years when I studied insertional mutagenesis
of chlamydomonas reinhardtii and developmental research
at the Debbie School for Handicapped Children, both
at the University of Miami . After joining Baylor College
of Medicine as a pediatric fellow in gastroenterology,
hepatology and nutrition, I met Dr. Burrin and began
a project focused on the influence of GLP-2 on intestinal
blood flow in July 2003. Specifically, I am investigating
how GLP-2 affects blood flow in the superior mesenteric
artery and within the mucosal layers of the small intestine
in the TPN-fed piglet. This information maybe helpful
in my clinical practice with children who are TPN dependent.
 Jeremy Cottrell, Ph.D.
B.Sc. (Hons), James Cook University,
Australia, 1997
Ph.D. Victoria University, Australia, 2003
I came from Australia to the Burrin
lab in October 2003 after a varied past, which started
with studies utilizing nuclear magnetic resonance (NMR)
and finished with stress physiology and meat science.
Within the Burrin lab, my research will investigate
GLP-2 mediated improvements in gut function during
total parenteral nutrition (TPN) induced villus atrophy.
While TPN is a life saving therapy, intestinal atrophy
is an unfortunate side effect. Previous research has
demonstrated that the gut hormone GLP-2 reduces intestinal
atrophy during TPN. My research will focus on whether
the improved intestinal morphology translates to improved
intestinal function, specifically transport of luminal
sugars.
 Rafal
Filip, M.D.
November, 2003 – March, 2004
M.D. Medical University School in Lublin, Poland
1996
I came to Doug Burrin's lab in November 2003 as
a visiting scientist. My current position is Head of
the Department of Metabolic and Degenerative Diseases
of Bone Tissue at Institute of Agricultural Medicine
in Lublin. The aim of my project in the Burrin Lab
is to quantify the metabolic fate of 13 C-labeled alpha-ketoglutarate
in the gut using the pig model. Most of my work focuses
on the determination of 13 C-AKG enrichment in plasma
samples using GC-MS. These studies may lead to future
studies examining the whether dietary supplementation
with AKG can prevent osteoporosis. |
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 Maaike
Riedijk, M.D.
October, 2003 – June, 2004
M.D. Medical School, University of Utrecht , 2000
Ph.D. Neonatology-Nutrition, Erasmus Medical MC
Sophia's Children's Hospital Rotterdam, 2003-current
I came to Doug Burrin's lab in October 2003 to work
on a collaborative project with my mentor, Johannes
Van Goudoever. The aim of the project is to determine
the metabolic fate of dietary methionine in the neonatal
gut using the infant pig model used in the Burrin Lab.
We are especially interested in establishing the role
of the gut in whole body transmethylation and transsulfuration
of dietary methionine. This work will become part of
my PhD dissertation thesis and will be combined with
similar clinical studies with methionine tracers in
preterm infants to be conducted at the Sophia Children's
Hospital.
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Harri
Niinikoski, Ph.D.
MD, University of Turku, Finland, 1991
PhD University
of Turku , Finland , 1997
Current address:
Department of Pediatrics
Turku University Hospital
Email: harri.niinikoski@tyks.fi
I received my medical training in pediatrics in
Finland . My PhD thesis focused on the role of serum
lipids and growth of children aged below three years.
After working for several years in the clinical world
with sick kids, I completely forgot how to handle a
pipetter. I came to Doug Burrin's Lab in August 2001
to study neonatal intestinal metabolism using the piglet
model. My aim is to investigate how intestinal blood
flow and substrate metabolism contribute to the TPN-induced
mucosal atrophy in neonatal piglets. I am also studying
the effects of GLP-2 on intestinal substrate metabolism
using this model. I'd like to understand the mechanisms
behind intestinal malfunction in severely ill children
and find ways to prevent it. After my postdoctoral
training, I will return to Finland and continue in
clinical practice and use my basic research experience
to establish a research program.
Publications:
Niinikoski, H, B Stoll, X Guan, K Kansagra, BD Lambert,
B Hartmann, J Stephens, JJ Holst, and DG Burrin. Onset
of small intestinal atrophy is associated with reduced
intestinal blood flow in TPN-fed neonatal piglets.
J Nutr (in press) |
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 Ketan Kansagra, M.D.
(July 2001-June 2003)
M.D., Jefferson Medical College, Philadelphia,
PA, 1997
B.S., The Penn State University,
University Park, PA 1995
Current address:
St. Peter's University Hospital
Division of Neonatology
New Brunswick , NJ
Email: ketank@psualum.com
I was a non-traditional student, completing my undergraduate
and medical training in six years. Maybe those two
years were important for unlike Harri (see Niinikoski,
Harri), I never learned how to use a pipette. I came
to Doug Burrin's Lab in February 2001 as a clinical
fellow in neonatology to study neonatal gut barrier
function using the piglet model. Over the last two
years, I have become more proficient and comfortable
in the lab. My aim is to answer the question, "Does
total parenteral nutrition impair neonatal gut barrier
function?" We have evaluated two components of gut
barrier function, permeability and bacterial translocation.
We are currently focusing on several cellular and molecular
factors that play a role in mucosal permeability. Upon
completion of my fellowship, I plan to obtain a position
as a clinician- educator at an academic institution.
Publications:
Kansagra, K., Stoll, B., Rognerud, C., Niinikoski,
H., Ou, C.N., Harvey , R., and Burrin, D.G. 2003. Total
parenteral nutrition adversely affects gut barrier
function in neonatal piglets. Am. J. Physiol. 285:G1162-G1170.
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 Barry Lambert, Ph.D.
(January 2002-June 2003)
B.S. Tarleton State University, Stephenville,
Texas, 1996.
M.S. Tarleton State University, Stephenville, Texas
, 1998.
Ph.D. Kansas State University, Manhattan, KS , 2001.
Current address:
Department of Animal Sciences
Tarleton State University
Email: blambert@tarleton.edu
My research interests relate to macronutrient metabolism
and identifying strategies to improve the efficiency
of nutrient use by animals. My Ph.D. thesis at Kansas
State University focused on hepatic methionine metabolism
and factors affecting the methionine metabolic pathway
in cattle. I came to Doug Burrin's Lab in January 2002.
The aim of my project is to establish the intestinal
metabolic fate of enteral alpha-ketoglutarate in neonatal
pigs. I am also studying the relative rates of glucose
and galactose metabolism by the gut and whole-body
in enterally fed and GLP-2 treated piglets.
Publications
Lambert, B, B Stoll, H Niinikoski, S Pierzynowski ,
and DG Burrin. 2002. Net portal absorption of enterally
fed "-ketoglutarate is limited in young pigs.
J. Nutr. 132: 3383-3386. |
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Lab Assistants |
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 Xiaofeng
Lu
BS from Biology, East China Normal University, Shanghai,
PR China, 1986
MS from Physiology, East China Normal University, Shanghai
PR China, 1992
MS from Microbiology, Montana State University, Montana,
USA, 1996
I have worked in the academic laboratories for about
ten years. My expertise consists of protein chemistry,
molecular biology and other laboratory techniques. I
joined Doug Burrin’s lab in the summer of 2000.
My responsibilities in the lab include developing new
methods, providing training and technical support for
the postdoctoral fellows and managing the routine laboratory
tasks. I have been closed involved in several ongoing
projects in the lab such as regulatory expression of
the intestinal matrix metalloproteinases (MMP) and nitric
oxide synthase (NOS) in the neonatal piglets.
Publications:
Lu, X., F. Roe, A. Jesaitis, and Z. Lewandowski, "Resistance
of Biofilms to the Catalase Inhibitor 3-amino-1,2,4-triazole
" Biotechnol. Bioengrg., 59(2):156-162,1998
Braha, O., L. Gu, L. Zhou., X.Lu, S. Cheley, H. Bayley
" Simultaneous Stochastic Sensing of Two Divalent
Metal Ions with a Single Engineered Protein Pore."
Nature Biotechnology 17, 1005-1007. 2000
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